Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • szy0931
    Member
    • Aug 2015
    • 51

    #1

    cuffdiff does not gene name

    Hi, all
    I am analyzing my data in Galaxy using bowtie2--cufflinks--cuffmerge--cuffdiff.
    I used the annotation gff file in the steps of cufflinks and cuffmerge.
    Now in the result of cuffdiff, I cannot find the gene names.
    My results in the sqlite file have such columns:
    gene _id sample_1 sample_2 status values_1 value_2 log2_fold_change test_stat p_value 1_value significant
    How can I let the result shows up gene name?
    Thank you!
  • szy0931
    Member
    • Aug 2015
    • 51

    #2
    update
    I just found the gff file I used does not have the column gene name though it has gene name described in the last column with other information.
    So, I think that is the reason

    Comment

    • szy0931
      Member
      • Aug 2015
      • 51

      #3
      update:
      I found a file of feature_table.txt has gene names. How can I convert the feature_table.txt file in to gtf or gff3 file?

      Comment

      • SylvainL
        Senior Member
        • Feb 2012
        • 180

        #4
        With the package rtracklayer for R it is quite easy. Import your table, make a GRanges object with the correct elementMetadata and then export in gtf...

        Comment

        Latest Articles

        Collapse

        • kinpict.com
          Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by kinpict.com
          The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
          Today, 06:37 PM
        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2





          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
          10-30-2026, 06:46 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 11-12-2026, 07:59 AM
        0 responses
        26 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-05-2026, 09:25 AM
        0 responses
        35 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-03-2026, 11:54 AM
        2 responses
        56 views
        0 reactions
        Last Post kinpict.com  
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        175 views
        0 reactions
        Last Post SEQadmin2  
        Working...