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Originally posted by sanwen
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I came across the same problem when running R in a linux server. How do you fix it?
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You can download the R file with some other means (wget, web browser), "cd" to the download directory and source it locally in R with
However, to install Bioconductor packages, you need a working internet connection. Contact your systems administrator and ask him/her to help you set up R with your proxy (you could also try what ioatomis did).Code:source("biocLite.R")Comment
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Thank U
Yes I've solved it, the problem is due to Web-limitation on that server nodes...but I didn't notice it at first...haha...thank U for your advice, it's really helped
Originally posted by sarvidsson View PostComment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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