Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • peromhc
    Senior Member
    • Sep 2009
    • 108

    #1

    optimizing transcriptome assembly

    Hi All,

    I wanted to make sure people have seen the new Oyster River Protocol for transcriptome assembly. In brief, it’s an evidence based protocol for optimizing assembly providing recommendations from the how many reads to generate, to how to report results, and everything in between. The protocol itself is here, the manuscript here, and the blog post here..

    Feel free to use and share with whoever, as you see fit. I’m happy to answer questions either here, or (preferably?), on Gitter https://gitter.im/macmanes-lab/Oyster_River_Protocol.
  • colindaven
    Senior Member
    • Oct 2008
    • 417

    #2
    Thank you for your efforts, this is very useful! Do you have any plans to distribute this via docker / VM ?

    Comment

    • peromhc
      Senior Member
      • Sep 2009
      • 108

      #3
      I had not thought to, but do you think that would be helpful for people?

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2





        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
        10-30-2026, 06:46 AM
      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Today, 07:59 AM
      0 responses
      13 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-05-2026, 09:25 AM
      0 responses
      32 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-03-2026, 11:54 AM
      0 responses
      45 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      169 views
      0 reactions
      Last Post SEQadmin2  
      Working...