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  • aleferna
    Senior Member
    • Sep 2009
    • 121

    #1

    Nimblegen Probes

    #chr10 NimbleScan TEST1/Ms-4C/gDNA/MseI 112111377 112111427 0.08 . . ;chr10P112111377;1
    #chr10 NimbleScan TEST1/Ms-4C/gDNA/MseI 112111477 112111527 0.31 . . ;chr10P112111477;1
    #chr10 NimbleScan TEST1/Ms-4C/gDNA/MseI 112111577 112111627 -0.19 . . ;chr10P112111577;1

    #CHR11 NimbleScan ESC/Mb-4C1/gDNA/Mbo1 11570417 11570467 1.26 + . CHR11:11425301-12332192;CHR1100P011570417;1
    #CHR11 NimbleScan ESC/Mb-4C1/gDNA/Mbo1 11570469 11570519 0.91 + . CHR11:11425301-12332192;CHR1100P011570469;1
    #CHR11 NimbleScan ESC/Mb-4C1/gDNA/Mbo1 11570521 11570571 1.67 + . CHR11:11425301-12332192;CHR1100P011570521;1


    I'm going through some old nimblegen tiling array gff files and they have 2 types of lines. The first show a single position on the genome (;chr10P112111577;1) and the second show a range (CHR11:11425301-12332192;CHR1100P011570469;1). I understand the second type, but what is the first?? I looked at the ndf file and it doesn't explain it.
  • cjfields
    Junior Member
    • Sep 2009
    • 6

    #2
    In the first example, the name can't be reporting a single position. It's more likely the specific probe ID. The number might represent the count; compare it to a recent set of GFF3 output I have:

    Code:
    ##gff-version	3
    AARF01000001	NimbleScan	data_371701C03_HX1_U01_Apis_532:BLOCK1	256	305	867.89	.	.	seq_id=AARF01000001:1-883;probe_id=AARF01000001FS000000256;count=1

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