While we sequencing CFTR with long-range PCR + Nextera XT + MiSeq 2x250bp, we detected homozygous variant in sample. (upper part of image)
However, when we sequenced the same sample with short-range PCR with same conditions, the variant seemed heterozygous. (lower part of image)
What could be the reason for that?
Is it possible that our long-range primers bind and amplify only one copy of CFTR?
However, when we sequenced the same sample with short-range PCR with same conditions, the variant seemed heterozygous. (lower part of image)
What could be the reason for that?
Is it possible that our long-range primers bind and amplify only one copy of CFTR?
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