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Does any one know why the number of junction reads of TopHat (117410) are very less compared to ERNAGE. It should be more than ERANGE I suppose ?
And the genomic reds number is more than the original reads? (32233301)
I ran Illumina solexa single end rnaseq reads
BLAT+ERANGE
Samples No. Reads Genomic reads Genomic reads Junction reads
Adipose 27752231 17.6 M (unique) 4.8 M (non-unique) 1.4MB
BOWTIE+TOPHAT
Samples No. Reads Genomic reads Junction reads
Adipose 27752231 32233301 117410
And the genomic reds number is more than the original reads? (32233301)
I ran Illumina solexa single end rnaseq reads
BLAT+ERANGE
Samples No. Reads Genomic reads Genomic reads Junction reads
Adipose 27752231 17.6 M (unique) 4.8 M (non-unique) 1.4MB
BOWTIE+TOPHAT
Samples No. Reads Genomic reads Junction reads
Adipose 27752231 32233301 117410
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