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  • steven
    Senior Member
    • Aug 2009
    • 269

    #16
    Originally posted by dukevn View Post
    Thanks! Very useful paper for my purpose. It looks like if the run is RNA-Seq (or miRNA-seq etc...), the % coverage should be really low (the paper said that > 93% of uniquely mapped reads fell into exon regions). In this case, our numbers are funny . Oh well, dont know what is happening here.
    It also depends on what kind of RNA you are working with (total RNA, polyA+ selected, cytoplasmic, nuclear, ribo-minus, etc). Plus this number of 93% is not a golden standard..
    You may be interested in these previous threads: here and there

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