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  • Niranjanks
    Member
    • Aug 2015
    • 11

    #1

    How important is a Read Group for GATK?

    Hello I am doing Variant Calling on a sample -

    I received a total of 3 files to analyse.
    They were divided into 2 runs
    So Run1 > 1 paired end file
    and Run2 > 2 paired end files run on different lanes

    GATK Best Practices Variant Calling pipeline required that I add Read Groups to the files which I did using Picard AddOrReplaceReadGroups.

    I tried merging the 3 files and then performed Marking Duplicates and this next step needs Read Groups. I can give it a single read group right now

    OR

    I can go back and assign 3 different read groups to the 3 set of files and then merge them

    What is preferable?
    Last edited by Niranjanks; 02-10-2016, 12:34 AM.

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