Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Niranjanks
    Member
    • Aug 2015
    • 11

    #1

    How important is a Read Group for GATK?

    Hello I am doing Variant Calling on a sample -

    I received a total of 3 files to analyse.
    They were divided into 2 runs
    So Run1 > 1 paired end file
    and Run2 > 2 paired end files run on different lanes

    GATK Best Practices Variant Calling pipeline required that I add Read Groups to the files which I did using Picard AddOrReplaceReadGroups.

    I tried merging the 3 files and then performed Marking Duplicates and this next step needs Read Groups. I can give it a single read group right now

    OR

    I can go back and assign 3 different read groups to the 3 set of files and then merge them

    What is preferable?
    Last edited by Niranjanks; 02-10-2016, 12:34 AM.

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Today, 07:59 AM
0 responses
13 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
32 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
0 responses
45 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
169 views
0 reactions
Last Post SEQadmin2  
Working...