I have a 50bp SE mouse miRNA-Seq study, I did quality and adapter trimming, aligned to miRBase v21,
% of uniquely aligned reads is 30-50% across samples, 40% average
about 17-30% aligns non-uniquely
25-50% are unmapped.
Just wondering if it sounds right, any comments form your experience in mice/human experiments.
Thanks
% of uniquely aligned reads is 30-50% across samples, 40% average
about 17-30% aligns non-uniquely
25-50% are unmapped.
Just wondering if it sounds right, any comments form your experience in mice/human experiments.
Thanks
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