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  • epi
    Member
    • Jan 2012
    • 38

    #1

    miRNA-Seq alignment rate

    I have a 50bp SE mouse miRNA-Seq study, I did quality and adapter trimming, aligned to miRBase v21,

    % of uniquely aligned reads is 30-50% across samples, 40% average
    about 17-30% aligns non-uniquely

    25-50% are unmapped.

    Just wondering if it sounds right, any comments form your experience in mice/human experiments.

    Thanks
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250

    #2
    Your figures are consistent with results that I have seen.

    Comment

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