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  • tonup69
    Associate Professor
    • Apr 2011
    • 20

    Cuffmerge Exon vs Transcript output from Cufflinks Problem.

    One issue I continue to have is that Cufflinks output contains both "transcript" and "exon" lines which, in some cases, match the same gene ID and coordinates. Cuffmerge is seeing this as an error (i.e. it sees duplicate genes and fails). I did a work around by selecting just exon level data for cuffmerge, but now my cuffdiff output does NOT have CDS tables (as expected). Can I still build a database for CummeRbund or is there some other work around I should use.

    Using updated Cufflinks and Cuffmerge tools 2.2.1.0 in our GALAXY install.

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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