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  • guilhem
    replied
    Thank GenoMax.
    I saw this interesting post before posting. I was just wondering before trying this if hisat2 can have natively this function since I saw it can trim and 'soft clip' -- which I thought was similar to clipping adapters.

    Leave a comment:


  • GenoMax
    replied
    Look at BBDuk.sh from BBMap. It should be intuitive to use and fast. You would want to process paired-end data files together if you have that kind of data.

    Leave a comment:


  • guilhem
    started a topic Clip adapter Hisat2

    Clip adapter Hisat2

    Hi,

    I am processing analysis on reads obtained from ribosome profiling experiments.
    I need first to clip adapter before mapping my reads.
    This step, however, is very time consuming with fastx_clipper.
    I am wondering if there is any other way to it faster, for instance directly into hisat2 would be awesome.
    Thanks for your advises,

    G

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