Each line represents a variant found in the DNA sequence, so if I specify a region present in the file the algorithm has to return that region.
It's strange not to work for the whole file.
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Where did you get the genome? ENSEMBL (names of chromosomes without "chr"), UCSC (then it should be "chr10")?
Does this function work if you request for example?
vc = file.query("10", 995, 48262)
edit: our posts got crossed...
Did you try to request a shorter sequence?
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Library Htsjdk to query a vcf file
I'm usingCode:VCFFileReader file= new VCFFileReader(file.vcf, file.vcf.gz.tbi, true)
Code:CloseableIterator<VariantContext> vc = file.query("10", 77162995, 131048262)
The problem is that when I choose a region in the middle or the end of the chromosome the method doesn't return anything. For the region starting at position 77162995 and end at 131048262 it doesn't work.Tags: None
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