Stringtie error

[tonup69]

Anybody know what this means? Still got assembled output, but can't use the files in Galaxy due to the error.

Warning: bundle FBgn0004047:1-1595(1089265) (224js) reached coverage saturation (1000000) starting with read mapped at 458

[GenoMax]

From StringTie:

-s coverage saturation threshold; further read alignments will be
ignored in a region where a local coverage depth of <maxcov>
is reached (default: 1,000,000);

You appear to be using an older version (current is v.1.2.2). Via galaxy?

[tonup69]

From StringTie:



You appear to be using an older version (current is v.1.2.2). Via galaxy?

Yes, I am using an older version on our local Galaxy install. I am not in charge of updating the box, though. I found a solution by increasing the max threshold for coverage saturation. Seems to work. I will not be able to use the new version until we work out some updating issues with BioTeam. Not very happy with them, actually.

Thanks for the quick reply.