strand bias

[Bioinfo26]

Hi All,
I have to check strand bias for 3 strains of C. trachomatis A, B,C genome , to perform strand bias and check the bias in which strand. Mostly its said, that bias occur in leading strand, I have to check in which strand in my 3 strains genome. any way to find most essential gene in these strain.. Any method to perform strand bias.. Using codonW, i have got some results, I have to compare these results with strand bias results and select most essential gene. please help me to perform..

Thanks in advance

[GenoMax]

Have you seen this thread: https://www.biostars.org/p/16033/

What kind of bias are you interested in? If you are referring to just strains of the same organism you would likely need experimental data depending on what kind of bias you are interested in. If you are simply referring to what strand the genes are encoded on then do you think there will be a difference in three strains of the same species?

[Bioinfo26]

I have to check GC skew and the bias which influence mechanism of gene.. How to find leading strand and lagging strand in whole genome? Any online tools are there