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  • boetsie
    Senior Member
    • Feb 2010
    • 245

    #1

    bowtie paired end mode

    hi all,

    i want to use bowtie for sort of paired end alignment, when i do;

    bowtie.exe TESTTT -c CACCGAAAGACA,AGTAGCCACGGCGCAGCAACAC

    it works fine, and i get two outputs.

    However, when i do them in paired end mode;

    bowtie.exe TESTTT -1 file1.txt -2 file2.txt

    it doesn't work, since it generates no outputs. Someone knows how to generate such a file? Can it be a .fasta file?

    Cheers,
    Marten
  • adamdeluca
    Member
    • Jul 2010
    • 95

    #2
    http://bowtie-bio.sourceforge.net/manual.shtml

    -f specifies fasta, by default fastq is assumed

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