I'm now analyzing MNase-seq data. But I'm confusing about the simulation method of MNase-seq reads. Because MNase has cut bias for AT-rich sequence. So I should simulate the sequencing data to mimic observed cleavage biases and length distribution. Is there any software that can be used to do this kind of simulation? Thank you very much!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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