Tweet
Differences between BED, WIG CEL and SRA
Hello!can anyone explain to me the differences between BED, WIG CEL and SRA files?thanks in advance!
-
-
BED format: https://genome.ucsc.edu/FAQ/FAQformat.html#format1Originally posted by VC87 View Post
WIG format: https://genome.ucsc.edu/goldenPath/help/wiggle.html
CEL files: http://media.affymetrix.com/support/...-agcc/cel.html
SRA format: http://www.ncbi.nlm.nih.gov/books/NBK158900/
BED format represents interval data. WIG format is used for displaying information. CEL files store intensity and are generated as a part of affymetrix chip scans. SRA files used by Short read archive at NCBI internally to store NGS data in a compressed format.Last edited by GenoMax; 03-02-2016, 05:02 AM. -
Thank you for your reply!Comment
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment