I am using bowtie and have been outputting .bwt files but now need to convert those files to .sam format. I know bowtie has a .sam output option (-S) which I am now using, however I need to convert all the files I have produced so far using the .bwt format into SAM files so I don't lose all the work I have done so far. Using bowtie2sam.pl I can easily convert the files but the new files don't have headers, which is I need for the SAM -> BAM conversion. Is there a way to convert the files that creates headers or would it be better to manually add new headers with a loop of some kind? Thanks for the help!
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by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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by SEQadmin2
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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