Hi,
I have data produced from the ScriptSeq mRNA-seq library prep kit and I just want to check that reads produced from this kit should be complement to the transcript and for analyzing the gene expression from this kit using htseq-count, the --stranded parameter should be set to --stranded=yes?
Thanks!
Leanne
I have data produced from the ScriptSeq mRNA-seq library prep kit and I just want to check that reads produced from this kit should be complement to the transcript and for analyzing the gene expression from this kit using htseq-count, the --stranded parameter should be set to --stranded=yes?
Thanks!
Leanne