I have a small dataset containing five genes and their expression at 20 different time points, I want to construct the transcriptional regulatory network based on the data. Are there any tools or tutorials available to do this? I would much prefer tutorials.
-
-
Do your genes have impact on each others? If I understand well, the only data you have are mRNA levels, no protein levels, etc...
If your genes are transcriptional regulators, and making some assumptions that the mRNA levels directly reflect the active form of the protein levels, then maybe it is possible (i actually had a project like that 15 years ago but we had the mutants of each gene so we could infer the regulatory network between 5 transcriptional factors)
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment