hi~ everybody,I don't know how the mate-paired reads work in your assembly things? Just as we have one reads,how you find its pair reads?
thanks
thanks
-
-
It depends on the format. Illumina produces two file s_1_1_sequences.txt and s_1_2_sequences.txt . The first read in the first file is in mate relationship with the first read in the second file.
Sometimes, some assemblers requires the mates in one after the other in the same file, but this depends on the assembler. -
thanks~ I understandComment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment