Tweet
hi~ everybody,I don't know how the mate-paired reads work in your assembly things? Just as we have one reads,how you find its pair reads?
thanks
thanks
-
-
It depends on the format. Illumina produces two file s_1_1_sequences.txt and s_1_2_sequences.txt . The first read in the first file is in mate relationship with the first read in the second file.
Sometimes, some assemblers requires the mates in one after the other in the same file, but this depends on the assembler. -
thanks~ I understandComment
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment