Hello everyone,
I am new here and have a very basic question please.
When someone is saying that a good library size for an RNA seq experiment is 30 M paired-end reads (e.g.: section III.2 of the ENCODE project's RNA standards, from the ENCODE experiment guidelines page), what does he/she mean please?
1- is 30 M reads the number of forward reads + the number of reverse reads?
2- is it the number of templates sequenced (i.e.: == the number of forward reads for instance)?
Many thanks for your help
I am new here and have a very basic question please.
When someone is saying that a good library size for an RNA seq experiment is 30 M paired-end reads (e.g.: section III.2 of the ENCODE project's RNA standards, from the ENCODE experiment guidelines page), what does he/she mean please?
1- is 30 M reads the number of forward reads + the number of reverse reads?
2- is it the number of templates sequenced (i.e.: == the number of forward reads for instance)?
Many thanks for your help
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