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  • AurelieMLB
    Junior Member
    • Apr 2016
    • 1

    #1

    Library size exact definition please

    Hello everyone,

    I am new here and have a very basic question please.

    When someone is saying that a good library size for an RNA seq experiment is 30 M paired-end reads (e.g.: section III.2 of the ENCODE project's RNA standards, from the ENCODE experiment guidelines page), what does he/she mean please?
    1- is 30 M reads the number of forward reads + the number of reverse reads?
    2- is it the number of templates sequenced (i.e.: == the number of forward reads for instance)?

    Many thanks for your help
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Paired end include forward + reverse reads. Each pair of reads sample the same fragment, so 15 M distinct fragments.

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