DArT seq vs GBS & ddRAD

[Jayakumar S]

Hi,
Can anyone update me about the difference between the DArT seq vs GBS & ddRAD.
DArT array is for studying the expression pattern.
Also let me know which method is found to be more useful for plants and livestock species.

Thanks in advance

Jayakumar S

[nucacidhunter]

DArTSeq protocol is not in public domain and it scores both SNPs and dominant markers (present/absent). I would be wary about dominant markers as absence of a fragment could be for other reasons than polymorphism and it cannot be correctly tested by segregation analysis.

GBS produces lots of tags with low coverage and lots of missing data points so it is most suitable for RILs.

ddRAD is tuneable and one can get good coverage for SNP analysis. They all are suitable for any organism and preference depends on application whether you are analysing crosses or population.