Convert fastq/qual to sff file

[SDPA_Pet]

Hi, does anyone know any tools can convert fastq (or fasta+qual) file to sff files.

I used to get 454 sequencing back as raw sff files. Now, they all give me fasta+qual file. I can easily make a fastq file from the two files. Is it possible to convert to sff file?

~Ben

[GenoMax]

See https://www.biostars.org/p/14594/

[SDPA_Pet]

See https://www.biostars.org/p/14594/

That's so complicated. I don't understand.

[GenoMax]

That's so complicated. I don't understand.

Not possible to do the conversion you are asking for because all information in SFF file is not captured by fastq/qual.

Easiest would be to go back to your provider and ask them to give you SFF files in addition to fastq.