Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • zwzhu
    Junior Member
    • Oct 2009
    • 5

    #1

    scripture problem

    i have one lane of Paired-end reads with the length of 90nt from RNA-seq. These reads were aligned to reference genome sequence with tophat (v 1.0.13) as described in the documentation of Scripture, all left process was according to the instruction. but no result output.
    Here is a snippit of output I see on my screen.

    java -jar scripture.jar -alignment Chromosome1_YPD.all.alignments.sorted.sam
    -out chr1.scriptureESTest.segments_1 -sizeFile chromosome1.sizes -chr chr1
    -chrSequence Chromosome1.fa -pairedEnd Chromosome1_YPD.paired.sorted.sam

    Using Version VPaperR3
    Computing weights..... upweighting? false weight: 1.0
    Computing alignment global stats for chromosome chr1
    Has pairs: true
    Has upweighting turned on: false
    Computing weights..... upweighting? false weight: 1.0
    AlignmentDataModel loaded, initializing model stats
    Computing alignment global stats for chromosome chr1
    model stats loaded, initializing model
    Built the model: 0.0 free memory: 12112784
    Loaded chromosome Sequence
    Segmenting accross graph
    Going to get read iterator to make graph with counts
    Got read iterator
    Made it through all reads
    Collapsed reads
    Decollapsed by introns
    Made first graph
    Got extended pieces
    Made second graph
    Done making spliced graph
    Got Simple paths...
    Estimated distribution
    Made path trees
    Done adding paired ends (if available)
    Done getting paths. Total: 0
    Done with local segmentation
    Done setting local rate in graph
    Done scoring paths.

    "Collapsed reads"
    "Decollapsed by introns"
    what these mean?

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
22 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...