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  • Seq_g
    Junior Member
    • Mar 2010
    • 6

    #1

    GEO(NCBI) database data integration

    Dear all,
    I would like to visualise raw ( All possible binding sites ) data from few literatures and since i don't have much knowledge about the data formate I got confused. Eg: GSM162004 ( since the experiment entries : GSE7065 from the .tar file is in .CEL (binary) format, i downloaded individual txt format)
    x1086_y1242_r2_Tag_2 89.25
    x1087_y1242_r2_Tag_2 149.25
    x1086_y1241_r2_Tag_10 141.25
    and
    GSM506211_090313_s_3_seq_WKK13.soa:
    1 36 - chr3 2635473
    1 36 + chrC 26323
    1 36 + chr3 4070645

    How can I convert them to gff/wig file? I made a guess like this
    GSM162004.gff
    r2 . tarray 2 26 89.25 . . Name=r2_Tag_2
    r2 . tarray 2 26 149.25 . . Name=r2_Tag_2
    r2 . tarray 10 34 141.25 . . Name=r2_Tag10

    GSM506211.gff:
    chr3 2635473 2635438 1
    chrC 26323 26358 1
    chr3 4070645 4070680 1

    Is that correct?? In both case I can see some unusual pattern . In the GSM162004 for the reference seq I have the values like 25S 5S Actin chloroplast chr1 chr2 gcBin03 gcBin04 gcBin05 ... and for GSM506211 the score is always 1. Could you please guide me?

    Thanking you in advance,
    seq_g

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