Calculate FPKM/RPKM manually

[BioTalk]

Hello All,

I would like to calculate FPKM and RPKM for the data on the basis of number of counts of reads. Does any one know the formula to calculate it manually?

Thank you,

[lcollado]

Hello,

The next 2 threads have a lot of information on RNA-seq and calculating expression levels (RPKM, ...).

Smile how to study differential expression?
RNA-seq output

Plus, a simple forum search for the work "rpkm" returns a gamma of threads on the subject.

I guess that this is a pretty basic question that could be added to i.seqanswers.com ( 0 results when searching for rpkm)

Cheers,
Leonardo