Using Trinity in iPlant Atmosphere. The instance already has Trinity. When I try to use it with this : Trinity --seqType fq --single ERR279688_1_74qual90.fastq --run_as_paired --JM 20G --no_bowtie
I get this error: CMD: /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/trinity-plugins/jellyfish/bin/jellyfish count -t 2 -m 25
-s 2958344137 --both-strands single.fa
/usr/bin/ld: cannot open output file /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/trinity-plugins/jellyfish/bin/.libs/8978-lt-jellyfish: Permissio
n denied
collect2: ld returned 1 exit statusnError, cmd: /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/trinity-plugins/jellyfish/bin/jellyfish count -t 2 -m
25 -s 2958344137 --both-strands single.fa died with ret 256 at /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/Trinity line 1958.
iPlant says they can do nothing. I tried sudo but then it says Trinity is not found. I tried downloading Trinity with both git clone and curl but then different errors come up. Any advice?
I get this error: CMD: /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/trinity-plugins/jellyfish/bin/jellyfish count -t 2 -m 25
-s 2958344137 --both-strands single.fa
/usr/bin/ld: cannot open output file /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/trinity-plugins/jellyfish/bin/.libs/8978-lt-jellyfish: Permissio
n denied
collect2: ld returned 1 exit statusnError, cmd: /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/trinity-plugins/jellyfish/bin/jellyfish count -t 2 -m
25 -s 2958344137 --both-strands single.fa died with ret 256 at /opt/bioinformatics_apps/trinityrnaseq_r20140413p1/Trinity line 1958.
iPlant says they can do nothing. I tried sudo but then it says Trinity is not found. I tried downloading Trinity with both git clone and curl but then different errors come up. Any advice?
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