What is RNA bait?

[surajit_micro]

Can anyone explain how to design RNA bait for whole genome sequencing?

[nucacidhunter]

I cannot work out why you would need RNA bait for whole genome sequencing. For RNA bait design for targeted sequencing following links might be useful:

http://www.mycroarray.com/mybaits/mybaits-custom.html

https://earray.chem.agilent.com/suredesign/

[atcghelix]

It can apparently be helpful for things like ancient DNA extractions where the percentage of exogenous DNA is high.

This is the paper I know of, and they have instructions of making the baits yourself (I've never tried it):

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824117/

[nucacidhunter]

Following is a link to aDNA sequencing using a predesigned capture kit:

http://www.mycroarray.com/mybaits/mybaits-WGE.html

or you have to follow that paper's method to produce your own baits.