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  • uselessAl
    Junior Member
    • Apr 2016
    • 4
    #1

    exploring 16s rRNA data

    Hey guys,

    I am wondering what the limitations of just having 16s rRNA data are for qiime analyses and how quantitative it could possibly be?

    Cheers
    Tags: None
    • fanli
      Senior Member
      • Jul 2014
      • 197
      #2
      First off, 16S amplicon sequencing is not quantitative. Your measurements are relative abundances. Other limitations:
      - difficult to resolve taxonomy beyond genera
      - differences in community profiling depending on primers used

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