Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • thedamian
    Member
    • Feb 2012
    • 50

    (SnpEff) SnpSift annotates only the first .vcf line

    Hi,
    I want to annotate my .vcf file based on ExAC database.
    I'm ONLY interested in AC_EAS tags from the database

    I do:
    java -jar SnpSift.jar annotate -info AC_EAS ExAC_db.vcf.gz my.vcf.gz > out.vcf

    however only the first variant in my.vcf.gz is annotated and the tag AC_EAS=0 is addad.
    All other positions remain empty, without annotations. These positions should be annotated since they exist both in my.vcf.gz and in ExAC_db.vcf.gz

    Did you have similar problem?

    Thx

Latest Articles

Collapse

  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 05:37 AM
0 responses
6 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
16 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
51 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-09-2026, 11:58 AM
0 responses
110 views
0 reactions
Last Post SEQadmin2  
Working...