New, I am working to assemble 454 (2.9G), Solexa pair-end (insert length = 400bp, read length = 120bp) and mate-pair (insert length = 3K and 5K, read length = 36bp). I have tried many software, velvet and mira, or methods in papers, but the results were not good. Is there any good method to assemble them.
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
