Seqanswers Leaderboard Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • probean
    Junior Member
    • Mar 2010
    • 6

    Split long fasta seq into smaller seqs by removing 'n'

    Hello,

    My fasta file has a long consensus sequence (gigabases long) that is padded with 'n' between the actual sequences. Like this:

    actgggacnnnnnnnnnnnnnnnnnnnnnnnnnnactgacgtggattgc
    aatnnnnnnnnnnnnnnnaccaattggatagagaccnnn

    I've searched rather intensively to see if the internet can solve my problem. Not really, though they were close. For example, people report splitting the long sequence into smaller files but I do not want that. I want the n removed and have all sequences in the same file. Better yet, with the start and end of the sequences.

    Desired output (>seqname_start_end) :
    >seq1_1_8
    actgggac

    >seq2_35_52
    actgacgtggattgcaat

    >seq3_68_85
    accaattggatagagacc

    If anyone could point me towards a right tool (bioperl, etc) or give me a pseudo-code in perl, I would appreciate it.

    Thanks.
  • azneto
    Member
    • Dec 2009
    • 24

    #2
    IDBA contains a script called split_scaffold

    Contribute to loneknightpy/idba development by creating an account on GitHub.


    Here's what it does:

    IN =>

    >test
    acgacgacgacgacgacgacgacagcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacgacgacgacagcagtagatgatgatagtag


    OUT =>

    >teste_0
    ACGACGACGACGACGACGACGACAGCA
    >teste_1
    ACGACGACGACAGCAGTAGATGATGATAGTAG

    Comment

    • probean
      Junior Member
      • Mar 2010
      • 6

      #3
      Originally posted by azneto View Post
      IDBA contains a script called split_scaffold

      Contribute to loneknightpy/idba development by creating an account on GitHub.

      Great! Thanks a lot! Worked flawlessly.

      It doesn't get me the start/end of the base positions but that I can live with.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Pathogen Surveillance with Advanced Genomic Tools
        by seqadmin




        The COVID-19 pandemic highlighted the need for proactive pathogen surveillance systems. As ongoing threats like avian influenza and newly emerging infections continue to pose risks, researchers are working to improve how quickly and accurately pathogens can be identified and tracked. In a recent SEQanswers webinar, two experts discussed how next-generation sequencing (NGS) and machine learning are shaping efforts to monitor viral variation and trace the origins of infectious...
        03-24-2025, 11:48 AM
      • seqadmin
        New Genomics Tools and Methods Shared at AGBT 2025
        by seqadmin


        This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.

        The Headliner
        The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...
        03-03-2025, 01:39 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-20-2025, 05:03 AM
      0 responses
      41 views
      0 reactions
      Last Post seqadmin  
      Started by seqadmin, 03-19-2025, 07:27 AM
      0 responses
      49 views
      0 reactions
      Last Post seqadmin  
      Started by seqadmin, 03-18-2025, 12:50 PM
      0 responses
      36 views
      0 reactions
      Last Post seqadmin  
      Started by seqadmin, 03-03-2025, 01:15 PM
      0 responses
      192 views
      0 reactions
      Last Post seqadmin  
      Working...