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  • doublealice
    Member
    • Feb 2011
    • 24

    mapping tool for short and long reads

    Hi,

    Please forgive if this had been asked before.

    I need one tool to map illumina short paired end reads (150bp) and Pacbio long reads to a genome. Usually I used bowtie2 for short reads, and blasr for Pacbio reads. But I have no idea if there is one tool work for both type of data.

    I found one paper used blat but can blat handle paired-end reads? Please give some suggestions. Thanks!
  • Michael.Ante
    Senior Member
    • Oct 2011
    • 127

    #2
    I'm sure bbmap can do that.
    STAR works very well with Illumina reads and the guys from Pacific Bioscience did some parameter optimisation to have it also working for PacBio reads (see here).

    Cheers,

    Michael

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      bwa mem also works with both short and long reads.

      Comment

      • doublealice
        Member
        • Feb 2011
        • 24

        #4
        Thanks Michael and Genomax. I will try both.

        Comment

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