Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • doublealice
    Member
    • Feb 2011
    • 24

    #1

    mapping tool for short and long reads

    Hi,

    Please forgive if this had been asked before.

    I need one tool to map illumina short paired end reads (150bp) and Pacbio long reads to a genome. Usually I used bowtie2 for short reads, and blasr for Pacbio reads. But I have no idea if there is one tool work for both type of data.

    I found one paper used blat but can blat handle paired-end reads? Please give some suggestions. Thanks!
  • Michael.Ante
    Senior Member
    • Oct 2011
    • 127

    #2
    I'm sure bbmap can do that.
    STAR works very well with Illumina reads and the guys from Pacific Bioscience did some parameter optimisation to have it also working for PacBio reads (see here).

    Cheers,

    Michael

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      bwa mem also works with both short and long reads.

      Comment

      • doublealice
        Member
        • Feb 2011
        • 24

        #4
        Thanks Michael and Genomax. I will try both.

        Comment

        Latest Articles

        Collapse

        • kinpict.com
          Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by kinpict.com
          The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
          Today, 06:37 PM
        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2





          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
          10-30-2026, 06:46 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 11-12-2026, 07:59 AM
        0 responses
        28 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-05-2026, 09:25 AM
        0 responses
        35 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-03-2026, 11:54 AM
        2 responses
        56 views
        0 reactions
        Last Post kinpict.com  
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        175 views
        0 reactions
        Last Post SEQadmin2  
        Working...