Hi,
I got a questions regarding the insert size in the sam files. In my understanding, the insert size is the size of the fragment measured by counting the distance between the starting locations of the pairend read. But sometimes, I notice there is negative insert size and sometimes a insert size over several thousand. How can I explain those insert size? I am using illumina pair end sequencing.
Thanks
I got a questions regarding the insert size in the sam files. In my understanding, the insert size is the size of the fragment measured by counting the distance between the starting locations of the pairend read. But sometimes, I notice there is negative insert size and sometimes a insert size over several thousand. How can I explain those insert size? I am using illumina pair end sequencing.
Thanks
Comment