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  • Madza Farias Virgens
    Junior Member
    • Oct 2016
    • 2

    #1

    How to decompress several fastq.gz files into one single file?

    How to decompress several fastq.gz files and concatenate them into one single .fastq file? There are two kinds of file in the same folder, some ending in ...R2_001.fastq.gz , and others ending in R1_001.fastq.gz. The work is to decompress and concatenate files ending in ...R2_001.fastq.gz into one R2_001.fastq file, and do the same for ...R2_001.fastq.gz files. Really appreciate any help. Thanks -madza
  • SNPsaurus
    Registered Vendor
    • May 2013
    • 525

    #2
    gunzip decompresses gz files, cat concatenates them, and * is a wildcard. This is all in the Unix command line once you've moved to the directory with the files.
    gunzip *R2_001.fastq.gz
    cat *R2_001.fastq > combined_R2_001.fastq
    Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

    Comment

    • dpryan
      Devon Ryan
      • Jul 2011
      • 3478

      #3
      Code:
      zcat *R2* > concatenated_R2.fastq.gz
      zcat *R1* > concatenated_R1.fastq.gz

      Comment

      • lindenb
        Senior Member
        • Apr 2010
        • 143

        #4
        cross posted https://www.biostars.org/p/215053/

        Comment

        • Madza Virgens
          Junior Member
          • Nov 2013
          • 1

          #5
          Would appreciate if someone with permissions can delete this for me.

          Would appreciate if someone with permissions can delete this for me.
          Didn't know crossposting was a prob. Thanks. -madza

          Comment

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