Tweet
Hello
I made an alignment with ncRNA.fasta sequence by Ensembl using tophat. however... in my output the trancript_ID stay in the chr local... So when I tried to run ht-seq count none sequence is found..
Why does it happen? there is a way to change information for chromosome or be compatible with ht-seq?
please, someone can help me??!!
HWI-7001432L:176:C8DRKANXX:6:2102:9302:3530 pUr1s ENSSSCT00000001325.2 5 1 76M * 0 0 GCCACCGCGGTTCGCGGTTCTAAACTCTCCATCCATTCGCCTCGACTCCGCTTCTCTCCAGACTCCGAGGCTGAGG BGGGGGFGGGGGGGDGDGGEGDGGGGGGGGGEGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCCCC AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:76 YT:Z:UU NH:i:3 CC:Z:ENSSSCT00000001326.2 CP:i:5 HI:i:0 HWI-7001432L:176:C8DRKANXX:2:1214:8451:68280 353 ENSSSCT00000000705.2 606 1 76M = 788 258 CTAAGTTTTCTTTCTTCTGGTTGGGATTATCATGGGATCCATCTGGCCAAAGGTGGCTGTGGGAAGATGGCACGCT BCCBCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGCGDFGGGGGGGGGGGGGGFGGEGGGGGGGG AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:76 YT:Z:UU NH:i:4 CC:Z:ENSSSCT00000033566.1 CP:i:641 HI:i:0
I made an alignment with ncRNA.fasta sequence by Ensembl using tophat. however... in my output the trancript_ID stay in the chr local... So when I tried to run ht-seq count none sequence is found..
Why does it happen? there is a way to change information for chromosome or be compatible with ht-seq?
please, someone can help me??!!
HWI-7001432L:176:C8DRKANXX:6:2102:9302:3530 pUr1s ENSSSCT00000001325.2 5 1 76M * 0 0 GCCACCGCGGTTCGCGGTTCTAAACTCTCCATCCATTCGCCTCGACTCCGCTTCTCTCCAGACTCCGAGGCTGAGG BGGGGGFGGGGGGGDGDGGEGDGGGGGGGGGEGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCCCC AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:76 YT:Z:UU NH:i:3 CC:Z:ENSSSCT00000001326.2 CP:i:5 HI:i:0 HWI-7001432L:176:C8DRKANXX:2:1214:8451:68280 353 ENSSSCT00000000705.2 606 1 76M = 788 258 CTAAGTTTTCTTTCTTCTGGTTGGGATTATCATGGGATCCATCTGGCCAAAGGTGGCTGTGGGAAGATGGCACGCT BCCBCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGCGDFGGGGGGGGGGGGGGFGGEGGGGGGGG AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:76 YT:Z:UU NH:i:4 CC:Z:ENSSSCT00000033566.1 CP:i:641 HI:i:0