Failed to write to file by trimgalore

[ruda2811]

I wanted to trimm by using trimglore but I'm doing something wrong and it doesn't work.
this is my script:

/home/weronika/Downloads/trim_galore_v0.4.2/trim_galore --illumina -q 20 -e 0.2 -length 43 -phred33 /home/weronika/Downloads/cfDNA/Project_Weronika/Sample_205/205_CCGTCC_L004_R1_001.fastq -o /home/weronika/Downloads/cfDNA/Project_Weronika/trimgalor/205_CCGTCC_L004_R1_001.fastq

and I get the answer
Path to Cutadapt set as: 'cutadapt' (default)
1.11
Cutadapt seems to be working fine (tested command 'cutadapt --version')
Failed to write to file '205_CCGTCC_L004_R1_001.fastq_trimming_report.txt': No such file or directory

I really don't know what to do. Please help me.

[ksawatzki]

I don't use this program, but try putting your -o command before the input:

/home/weronika/Downloads/trim_galore_v0.4.2/trim_galore --illumina -q 20 -e 0.2 -length 43 -phred33 -o /home/weronika/Downloads/cfDNA/Project_Weronika/trimgalor/205_CCGTCC_L004_R1_001.fastq /home/weronika/Downloads/cfDNA/Project_Weronika/Sample_205/205_CCGTCC_L004_R1_001.fastq

[wdecoster]

https://www.biostars.org/p/218527/

[HESmith]

...try putting your -o command before the input

The -o flag is for the output, not input.

[ruda2811]

wdecoster Thank you very much for your help.

[Dean29]

Nice! Thank you man for this!