Very simple question, whats the preferred method to remove peaks from analysis that are associated with single replicates only? Thanks.
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I'm sure there are a lot of ways to do it. In the past, I've just loaded the peak file into an R GRanges format and removed any peaks that didn't show any overlaps in findOverlaps(). You can change the parameters to find overlaps of certain lengths as well. But I don't actually know how kosher this is or if there other tools that would make this easier.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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