Hello,
I have some 2x300bp sequencing data and I want to treat it as if it were a 2x100bp run.
So, I have some paired-read FastQs with reads of all different lengths, up to 300bp. I want to trim them so that they are all less than or equal to 100bp in length.
Is this possible and what would be the easiest way to do it?
Alternatively if the longer reads could be cut up so they are split into different reads of roughly 100bp (e.g. 200bp read gets cut into two 100bp reads), that would be even better, but I'm not sure of this is possible.
Very many thanks for any help.
I have some 2x300bp sequencing data and I want to treat it as if it were a 2x100bp run.
So, I have some paired-read FastQs with reads of all different lengths, up to 300bp. I want to trim them so that they are all less than or equal to 100bp in length.
Is this possible and what would be the easiest way to do it?
Alternatively if the longer reads could be cut up so they are split into different reads of roughly 100bp (e.g. 200bp read gets cut into two 100bp reads), that would be even better, but I'm not sure of this is possible.
Very many thanks for any help.
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