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Trim Galore! removing specific adapters not present in reads
Hi, I fed a specific custom adapter sequence to trim galore on paired-end illumina files (~3B bp each), and it removed approx. 7M bases from each (--length... -
Deblur, Unknown variant, unable to interpret PHRED
Hey guys,
I am having trouble using deblur --worflow on all of my fastq data.
when using --workflow, with just an fastq... -
Trimming stringency w/ excessive data
I'm in the process of assembling a 500Mb animal genome and have both Illumina and PacBio data to work with using a hybrid assembly approach. Generally...Last edited by adamrork; 12-12-2019, 08:49 PM. -
QIAseq FX DNA Library Kit adapters for trimming
Can anyone point me to the sequences of QIAseq FX DNA Library Kit for adapters trimming in preprocessing? Illumina has exact sequences for trimming included... -
condetri illumina sequences trimming
Hello!
I'm new in bioinformatics and I'm trying to launch the program condetri for the quality trimming of my paired end illumuna sequences. in... -
Quality Trimming read loss - Trimmomatic
Hello community,
I plan to do a reference assisted genome assembly for a yeast genome.
I have some questions regarding the trimming... -
Understanding FastQC output before Vs after trim_galore
I am very new to genome assembly and teaching myself about pre-assembly QC steps.
I performed fastQC analyses on HiSeq4000 data for forward... -
ddRAD, STACKS, and Trimming
Please disregard/delete.
Last edited by Carcharodon; 07-13-2017, 04:51 PM. -
How do i trim multiple adapters from my RNAseq reads?
Hi all,
I am using Galaxy and I want to remove the universal adapters as well as the index adapters in each data file of my RNAseq data.... -
Merging fastQ files and trimming advice
Hello everyone,
I am new to using NGS techniques and the bioinformatics tools for analysing the acquired data so I hope I can get some... -
FastQ: trimming paired reads down to a set length
Hello,
I have some 2x300bp sequencing data and I want to treat it as if it were a 2x100bp run.
So, I have some paired-read... -
Primer filtering with bbduk / bbduk2.sh
Hello,
I have merged Illumina reads and try to filter out all reads that have both, a recognisable fwrd and a recognisable reverse primer... -
Can I remove index adapters after assembly?
I assembled a reference transcriptome de novo using Trinity with 2x300bp Illumina MiSeq PE reads. I used libraries from 4 individuals that were multiplexed... -
Quality trimming & filtering illumina reads
Hi,
I have a illumina MiSeq data set, 32GB size genome, 300bp reads. Quality of reads degrades towards the 3' end in both R1 & R2,... -
demultiplex FastQ files based on read names
Hi everybody,
my name is Fabian Roger and I am a PhD student in Ecology at the university of Gothenburg.
I am at a stage...
