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  • papori
    Senior Member
    • Dec 2010
    • 181

    #1

    DAVID significant cluster, with non significant cluster

    Hi all,
    I used David for RNA seq analysis and i am confused by understanding its output.
    The input for David is a list of gene symbol and the output for the clustering analysis is this for example:

    Annotation Cluster 4 Enrichment Score: 5.027140988398066
    Category Term Count % PValue Genes Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR
    GOTERM_BP_FAT GO:0030030~cell projection organization 48 5.217391304 6.49E-08 749 368 13528 2.355836768 1.86E-04 1.86E-04 1.16E-04
    GOTERM_BP_FAT GO:0007411~axon guidance 10 1.086956522 0.137379774 749 107 13528 1.687982731 1 0.809464215 92.92162514


    The analysis that we chose is by the GO.
    These are only partial results.
    The cluster includes 13 GO's in total and those i showed are the first and the last.
    So,both are in the same cluster, the first is significant, but the second isnt..?
    and I really want to use the second
    How should i treat it?

    Thanks,
    Pap
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    I think you know that you can't treat the axonal guidance group as significant.

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