Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • papori
    Senior Member
    • Dec 2010
    • 181

    DAVID significant cluster, with non significant cluster

    Hi all,
    I used David for RNA seq analysis and i am confused by understanding its output.
    The input for David is a list of gene symbol and the output for the clustering analysis is this for example:

    Annotation Cluster 4 Enrichment Score: 5.027140988398066
    Category Term Count % PValue Genes Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR
    GOTERM_BP_FAT GO:0030030~cell projection organization 48 5.217391304 6.49E-08 749 368 13528 2.355836768 1.86E-04 1.86E-04 1.16E-04
    GOTERM_BP_FAT GO:0007411~axon guidance 10 1.086956522 0.137379774 749 107 13528 1.687982731 1 0.809464215 92.92162514


    The analysis that we chose is by the GO.
    These are only partial results.
    The cluster includes 13 GO's in total and those i showed are the first and the last.
    So,both are in the same cluster, the first is significant, but the second isnt..?
    and I really want to use the second
    How should i treat it?

    Thanks,
    Pap
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    I think you know that you can't treat the axonal guidance group as significant.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
      by SEQadmin2



      Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
      ...
      07-09-2026, 11:10 AM
    • SEQadmin2
      Cancer Drug Resistance: The Lingering Barrier to Rising Survival
      by SEQadmin2



      Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

      There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
      07-08-2026, 05:17 AM
    • GATTACAT
      Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by GATTACAT
      Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
      07-01-2026, 11:43 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 07-13-2026, 10:26 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-09-2026, 10:04 AM
    0 responses
    32 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-08-2026, 10:08 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-07-2026, 11:05 AM
    0 responses
    34 views
    0 reactions
    Last Post SEQadmin2  
    Working...