Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • DAVID significant cluster, with non significant cluster

    Hi all,
    I used David for RNA seq analysis and i am confused by understanding its output.
    The input for David is a list of gene symbol and the output for the clustering analysis is this for example:

    Annotation Cluster 4 Enrichment Score: 5.027140988398066
    Category Term Count % PValue Genes Total Pop Hits Pop Total Fold Enrichment Bonferroni Benjamini FDR
    GOTERM_BP_FAT GO:0030030~cell projection organization 48 5.217391304 6.49E-08 749 368 13528 2.355836768 1.86E-04 1.86E-04 1.16E-04
    GOTERM_BP_FAT GO:0007411~axon guidance 10 1.086956522 0.137379774 749 107 13528 1.687982731 1 0.809464215 92.92162514


    The analysis that we chose is by the GO.
    These are only partial results.
    The cluster includes 13 GO's in total and those i showed are the first and the last.
    So,both are in the same cluster, the first is significant, but the second isnt..?
    and I really want to use the second
    How should i treat it?

    Thanks,
    Pap

  • #2
    I think you know that you can't treat the axonal guidance group as significant.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    17 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    43 views
    0 likes
    Last Post seqadmin  
    Working...
    X