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Hi, golharam and simonandrews
Thanks! I am using WinXP. I will try to run SeqMonk on my desktop first to see if it works.
-c
Thanks! I am using WinXP. I will try to run SeqMonk on my desktop first to see if it works.
-c
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This week I discovered that using BigWig files for coverage data works really nicely in GBrowse.
I would really love to have a simple plotting tool to take per chromosome coverage data from something like a BigWig file and plot a nice looking coverage figure. I'm thinking of coding it myself in (probably) python or (maybe) R but if anyone can point me to examples to get started that would be great. Although some genome browsers make coverage plots, they just don't look nice for publication use IMHO.Last edited by natstreet; 10-01-2010, 08:10 AM.Comment
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Hi, simonandrews
I just noticed that the SeqMonk Release Notes talks about SeqMonk v0.12.0 but it seems I can only find SeqMonk v0.11.0 online. Do you know where to find v0.12.0?
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I can see v0.11.0 listed at their website and if you swap 11 to 12 in the download URL there is something there to be downloaded
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Sorry, I'd failed to copy over the new version of the download page from our staging server. If you try again now the links should be in place (if you don't see them press shift+refresh in your browser to force a cache update).Originally posted by cliff View Post
Simon.Comment
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IGV scripting function
Use IGV to output plots of a region of a bam file with the following sample script
attached is an example (note path to bam and snapshotDirectory need to be absolute not relative)
new
load myfile.bam
snapshotDirectory mySnapshotDirectory
genome hg18
goto chr1:65,289,335-65,309,335
sort position
collapse
snapshotComment
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<shameless plug>Originally posted by arnoldliao View PostUse IGV to output plots of a region of a bam file with the following sample script
attached is an example (note path to bam and snapshotDirectory need to be absolute not relative)
new
load myfile.bam
snapshotDirectory mySnapshotDirectory
genome hg18
goto chr1:65,289,335-65,309,335
sort position
collapse
snapshot
Note that BEDTools has a tool called bedToIGV that will automatically create an IGV batch script from a BED, GFF, or VCF file.
</shameless plug>Comment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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