Tweet
I have a need to assign dispersed individuals back to a clutch of eggs. I have many samples from each clutch of eggs. Rather than conducting a sibship analysis between the eggs and the dispersed adults, would it be possible to homogenize a batch of eggs and sequence them as one sample that approximates the parental genomes and then conduct a parentage analysis to assign individuals back to their clutch?
-
-
For reference cross-posted: https://www.biostars.org/p/221930/
If is ok to cross-post on SeqAnswers. -
Sounds like a reasonable approach to me. The clutch presumably has paternal and maternal alleles, while a single individual will have half of the alleles present in the clutch, but I think the relationship would be clear unless the different clutches are highly similar.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.comComment
-
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM -
Studying ecosystems means dealing with complex, multi-species communities that are hard to observe at scale. This complexity, however, hides many important questions to be answered, from how biogeochemical cycles work and how climate change can affect species distribution to how conservation strategies can work best.
Genomics, particularly since the expansion of NGS, has transformed ecosystem ecology. By sequencing environmental DNA, we can now assess biodiversity without direct...05-06-2026, 09:04 AM
Comment