Error in maker (gene prediction)

rajesh gazara

Junior Member

Join Date: Sep 2013

Posts: 2
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#1

Error in maker (gene prediction)

11-25-2016, 11:30 AM

Hello Everyone,

Does anyone know about below error in maker annotation :
ERROR: Non-unique top level ID
While this is technically legal in GFF3, it usually
indicates a poorly fomatted GFF3 file (perhaps you
tried to merge two GFF3 files without accounting for
unique IDs). MAKER will not handle these correctly.

I used fasta file only with one contig. I don't know why I am getting Non-unique ID error.

If anyone know please reply. I am waiting for responses.
Thanking you in advance!

Regards,
Rajesh
Tags: maker, non-unique id
colindaven

Senior Member

Join Date: Oct 2008

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#2

12-02-2016, 02:28 AM

It seems you have included a GFF3 file in your maker options - one of your conf files. I would recommend supplying evidence as FASTA files to annotate your genome with, so make sure all the GFF3 files in the options are commented out.
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rajesh gazara

Junior Member

Join Date: Sep 2013

Posts: 2
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#3

12-02-2016, 04:41 AM

Hi colindaven,

Thanks for your reply. I made it.
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