Blastp SSL call error

[lbroetto]

Hi, I have undergone the follow error in Blastp standalone running in Ubuntu 16.04, and I don't know how to solve it, please any help is welcome. Thanks.

blastp -query Temp/Blastp_BbassianaXcupin_hits.txt -db nr -evalue 1e-12 -max_target_seqs 6 -seg yes -remote -word_size 6 -outfmt 6 -out Temp/Teste
Critical: [blastp] Secure socket layer (SSL) has not been properly initialized in the NCBI Toolkit. Have you forgotten to call SOCK_SetupSSL()?
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (310.5) [blastp] [blast4] Service not found
Error: (315.2) [blastp] CConn_Streambuf::CConn_Streambuf(): NULL connector: Unknown
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (310.5) [blastp] [blast4] Service not found
Error: (315.2) [blastp] CConn_Streambuf::CConn_Streambuf(): NULL connector: Unknown
Error: (802.5) [blastp] CObjectOStream: error at byte 118: stack is empty: write fault
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (310.5) [blastp] [blast4] Service not found
Error: (315.2) [blastp] CConn_Streambuf::CConn_Streambuf(): NULL connector: Unknown
Error: (802.5) [blastp] CObjectOStream: error at byte 118: stack is empty: write fault
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (303.7) [blastp] [URL_Connect; https//www.ncbi.nlm.nih.gov:443/Service/dispd.cgi?service=blast4&address=ldm-270E5K(127.0.1.1)&platform=x86_64-pc-linux-gnu] Failed to connect: Not supported
Error: (310.5) [blastp] [blast4] Service not found
Error: (315.2) [blastp] CConn_Streambuf::CConn_Streambuf(): NULL connector: Unknown
Error: (802.5) [blastp] CObjectOStream: error at byte 0: stack is empty: cannot close output stream
Error: (CIOException::eFlush) [blastp] COStreamBuffer::Flush: failed
Error: (CSerialException::eIoError) [blastp] byte 0: cannot close output stream
Error: (802.4) [blastp] Cannot close serializing output stream(CSerialException::eIoError) byte 0: cannot close output stream
Error: (802.5) [blastp] CObjectOStream: error at byte 118: stack is empty: write fault

[GenoMax]

NCBI has switched to using "https-only" connections. That is only supported in versions of blast+ package v.2.5 onwards. You should upgrade your blast to the latest (v.2.6 as of now) available.

[lbroetto]

Hi GenoMax, thanks for your answer. Upgrade was the first thing I have done (also I have made the Ubuntu upgrade), but still doesn't work. I have installed the latest Blast Standalone available (v.2.6).
Do you think I'm doing something wrong?
Thanks again.

[lbroetto]

GenoMax, I just did run the blastp help command and it answer me that even I have installed the 2.6.0+, actually the version 2.2.31+ is still running. I have just downloaded the 2.6.0 version and installed aside the older version with tar zxvpf command. How can I uninstall the older version and make the last one working?
Thanks again.

[lbroetto]

GenoMax you was right, actually I haven't uninstalled the older version. Now I did it right and installed the latest 2.6.0 version and the problem was solved, but in order to run I need to use ./blastp, Do you know why is it happening, if I try to run just blastp it say program is not installed.
Thanks.

[GenoMax]

Looks like you got that sorted out.

You can add the new location of the directory where you have the latest blast+ programs to your $PATH (something along the lines of export PATH=$PATH:/path_to_new_blast). To make the change permanent see this link.

[nmirza]

GenoMax you was right, actually I haven't uninstalled the older version. Now I did it right and installed the latest 2.6.0 version and the problem was solved, but in order to run I need to use ./blastp, Do you know why is it happening, if I try to run just blastp it say program is not installed.
Thanks.

@lbroetto
I am facing a similar problem. Can you please tell me how to uninstall the ncbi-blast-2.5.0+ version, I am having a hard time finding the right answer. I am using Ubuntu16.04 LTS.

[GenoMax]

@lbroetto
I am facing a similar problem. Can you please tell me how to uninstall the ncbi-blast-2.5.0+ version, I am having a hard time finding the right answer. I am using Ubuntu16.04 LTS.

Instead of worrying about uninstalling the old version just make sure only the new version is in your $PATH (or provide full path to the new blast executables in your command).