Hi, I am new to RNA-seq analysis and bioinformatics in general. I have been able to generate several plots using cummerbund in Rstudio however when I try to create a dispersion plot I get the following error. The input files were created with Cuffdiff version 2.2.1.3. Any ideas on how to get around this?
> setwd("/home/fgb2/Desktop/diff_out3/")
> library(cummeRbund)
> cuff_data <- readCufflinks('/home/fgb2/Desktop/diff_out3/')
> genes(cuff_data)
CuffData instance with:
107594 features and 6 samples
> disp <- dispersionPlot(genes(cuff_data))
> disp
Error: Faceting variables must have at least one value
>
> setwd("/home/fgb2/Desktop/diff_out3/")
> library(cummeRbund)
> cuff_data <- readCufflinks('/home/fgb2/Desktop/diff_out3/')
> genes(cuff_data)
CuffData instance with:
107594 features and 6 samples
> disp <- dispersionPlot(genes(cuff_data))
> disp
Error: Faceting variables must have at least one value
>
