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Hello All,
I have a strategy question related to BAC annotation. I have 500 BAC sequences from a single maize line to annotate. I am faced with a strategy dilemma.
1) Put all the BAC sequences in one file, create a multi-fasta file and run the annotation.
2) Go about the annotation on a per BAC basis, One BAC sequence per file.
I would like to know the pros and cons of both strategies if anyone has done such a thing. What i want to know is that when we run a set of evidences on a BAC multi-fasta file, is the mapping of a particular evidence to a particular BAC, dependent on the probability of its mapping to other BACs in the multi-fasta file.
Thank you all
Abhijit
I have a strategy question related to BAC annotation. I have 500 BAC sequences from a single maize line to annotate. I am faced with a strategy dilemma.
1) Put all the BAC sequences in one file, create a multi-fasta file and run the annotation.
2) Go about the annotation on a per BAC basis, One BAC sequence per file.
I would like to know the pros and cons of both strategies if anyone has done such a thing. What i want to know is that when we run a set of evidences on a BAC multi-fasta file, is the mapping of a particular evidence to a particular BAC, dependent on the probability of its mapping to other BACs in the multi-fasta file.
Thank you all
Abhijit