Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • rajeshkmaurya08
    Member
    • Feb 2017
    • 14

    #1

    To find the difference in base pair position of two fasta file

    Hello,
    I am just beginner in Perl,
    I have Two fasta file of different length.
    I would like to align them to find difference in nucleotide position.

    Output should be like this
    Total length of fasta files
    First reference file: 1253630 base pair
    Second file: 4523366 base pair
    If match 2nd file is same as 1st reference file.
    Match position at base pair
    a-t 455222
    c-g 455665
    if not match out put should like this
    Mismatch position at base pair
    A-C 100025
    C-T 600045
    Result: should be in Output.txt

    I tried this code given below:

    use strict;
    use warnings;
    my $file1 = 'chr20.txt';
    my $file2 = 'chr21.txt';
    my $error = 'error.txt';
    open(my $in1, '<', $file1) or die "Cannot open file '$file1' for readi
    +ng: $!";
    open(my $in2, '<', $file2) or die "Cannot open file '$file2' for readi
    +ng: $!";
    open(my $out, '>', $error) or die "Cannot open file '$error' for writi
    +ng: $!";
    my $lineno = 1;
    while (my $line1 = <$in1>)
    {
    my $line2 = <$in2>;
    print "$. : $line2,$line1,";
    printf $out "Error:lineno:%d mismatch found \n", $lineno
    unless $line1 eq $line2;

    ++$lineno;
    }
    close $out or die "Cannot close file '$error': $!";
    close $in2 or die "Cannot close file '$file2': $!";
    close $in1 or die "Cannot close file '$file1': $!";
  • rajeshkmaurya08
    Member
    • Feb 2017
    • 14

    #2
    can anybody help me to short out the problem?

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      Yesterday, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    25 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    15 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    71 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...